Biodegradation of Acetamiprid by both free and immobilized Lysinobacillus macrolides strain MSR-H10 in soil

Abstract

Neonicotinoids are a commercial insecticides used worldwide in agriculture and are one of the major environmental pollutants. The prospective toxicity of the residues present in environment to humans has expected considerable attention. In this study, fifteen bacterial isolates were isolated from the soil sample, and tested for their Acetamiprid degrading capacity on MSM media containing Acetampirid as the sole carbon source. Lysinobacillus macrolides strain MSR-H10 was identified using 16S rRNA as one on the best performing isolates. The effects of pH, temperature and agitation speed on the degradation efficiency of acetamiprid were investigated. Results indicated that L. macrolides is able to grow at different pH, temperature and agitation speed. Interestingly, immobilized bacteria with sodium alginate recorded highest performance for growth compared to free bacteria and was able to grow in two types of soil containing different concentration of acetampirid. The immobilized bacteria were found to be used efficiently for the degradation of acetamiprid consecutively without any decrease in their efficiency. Hence, L. macrolides has a great efficiency for the degradation of acetampirid at concentration 5 mg/L after 21 days from incubation period in clay soil.