GETTING AND CHARACTERISTICS OF COPROPORPHYRIN CONJUGATES OF MONOCLONAL ANTIBODIES

Abstract

Conjugates of Pd (II) coproporphyrin I N-hydroxysuccinimide ester with ferritin-specific monoclonal antibodies of the IgG2a subclass were obtained in a one-step conjugation reaction, with antigen-binding activity increased by 3-7 times. The two-phase dependence of the functional activity of the conjugates on the depth of modification indicates the presence of two populations of modified amino groups. Modification of the more accessible ones leads to functional activation of the IgG molecule. Advantages in binding to an antigen of modified monoclonal antibodies HSF 102 compared to native monoclonal antibodies are shown.