Fluorescence in situ hybridization detection of chromosome translocations induced by ~(60) Coγ-rays in human lymphocytes dose-response curve and persistence

Abstract

Objective\ To study dose\|response relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60 Co γ rays.Methods\ The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4\+# and 7\+# combination of composite whole chromosome\|specific DNA probes and Giemsa stain.Results\ The dose\|response relationship for chromosome translocation induced by 60 Co γ rays in vitro could be described by the function:Y=0.0030+0 0134D+0 0165D\+2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γ\|irradiation did not diminish over time,so it exhibited excellent persistence.Conclusions\ The results indicate that retrospective dose\|reconstruction can be accomplished using chromosome translocation frequency.