Flavonoid Compound from Rambutan Bark (Nephelium lappaceum L.)

Abstract

: Flavonoids included polyphenol compounds that are found in many plants which are important compounds in human food.The flavonoid group, including flavanone, flavone, dihydroflavonol, catechin, flavonol, flavan-3-ols, isoflavones, auron, anthocyanidins, proanthocyanidins and chalcones, has a general structure of C6-C3-C6. The aims of this study is to isolate and identify the group of flavonoid compound contained in rambutanbark ( Nephelium lappaceum L.). Rambutan bark powder was extracted maceration with methanol. Methanol extract was dissolved with ethyl acetate repeatedly until the solution was negative flavonoids. Ethyl acetate extract was dissolved with methanol and partitioned with n -hexane. The methanol extract which is a total of flavonoids separated by column chromatography using chloroform: methanol (90:10; 80:20 and 70:30 (v/v)). The isolates obtained were purified by preparative thin layer chromatography and producing flavonoid glycoside, yellow amorphous with Rf value of 0.25 using the chloroform: ethyl acetate eluent (50:50) v/v. The pure isolate obtained were analyzed by UV-Visible Spectropometer, FT-IR, and H-NMR. Based on the interpretation of spectroscopic data, the flavonoid compound isolated from rambutan bark wasflavonol group.