In vitro transfer of N-acetylglucosamine to endogenous glycoprotein acceptors catalyzed by the nucleus and the cytoplasmic membranes prepared from L1210 cells.

Abstract

The non-nuclear membranes and the nuclei prepared from L1210 cells catalyze the in vitro transfer of N-acetyl(14C)glucosamine from UDP-N-acetyl(14C)glucosamine to endogenous glycoprotein acceptors. Adequate analysis of these acceptors have demonstrated that the nucleus has its own N-acetylglucosaminyltransferase system that leads to the formation of N-N'-diacetylchitobiosylated proteins.