Confocal Microscopy

Abstract

"We are now able to take a series of photographs at slightly different focal planes or levels through a single cell or group of cells. These photographs ­result in a consecutive record of the internal architecture of the cell or cells. This accomplishment we have called "optical sectioning". ... By means of this new development a transparent specimen such as a group of cells may be sectioned optically. ... Detail above or below the focal plane does not ­interfere." [1]