SINTESIS SENYAWA 4’-HIDROKSI-4-METOKSIKALKON DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI KONTAMINAN PRODUK DARAH

Wiwit Sepvianti, Serafica Btari Christiyani Kusumaningrum
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Abstract

Despite of the blood products are crucial for health, it cannot be separated from the risks of bacterial contamination. The bacterial contamination obtained from collecting and processing blood processing. Various species of positive and negative Gram bacteria are known to be bacterial contaminants in blood products such as thrombocyte concentrate, packed red cell, dan whole blood. Therefore, it is essential to find an antibacterial compound that could inhibited the growth of bacterial contaminant in blood products. Chalcone compounds are known to become one of antibacterial since they have an unsaturated-ketone-group which acts as an antibacterial because it can damage the bacterial cell wall. The presence of hydroxyl group has important role for inhibiting bacterial contaminant growth as well. In this research, 4’-hydroxy-4-methoxychalcone was synthesized through the Claisen-Schmidt condensation reaction. The characterization of the chalcone compounds was carried out by mass spectroscopy using gas chromatography-mass spectroscopy (GC-MS) and identification of functional groups using Fourier transform infrared (FTIR). Bacterial contaminants from blood products that were used: Acinetobacter, Escherichia coli, Bacillus sp, and Staphylococcus aureus. In addition, Staphylococcus aureus from the American Type Culture Collection (ATCC) was used as comparing isolates. The antibacterial activity was analyzed by inhibition area method. The results showed that 4’-hydroxy-4-methoxychalcone successfully synthesized with the reaction product in yellow crystal formation with yield of 72,80%, relative purity 100%, and m/z 254. Antibacterial activity of chalcone compounds at various concentrations of 6.25 ppm; 12.5 ppm and 25 ppm against Gram-positive and Gram-negative bacteria are still relatively weak because the percentage of inhibition ability achieved is below 50%.
合成化合物4 ' -羟基-4-代谢骨科,测试血液产品污染物抗菌活性
尽管血液制品对健康至关重要,但它不能脱离细菌污染的风险。从采集和处理血液过程中获得的细菌污染。已知各种阳性和阴性革兰氏菌是血液制品中的细菌污染物,如凝血细胞浓缩物、填充红细胞和全血。因此,找到一种能够抑制血液制品中细菌污染物生长的抗菌化合物是至关重要的。查尔酮化合物被认为是一种抗菌剂,因为它们有一个不饱和酮基团,它可以破坏细菌的细胞壁,起到抗菌的作用。羟基的存在对抑制细菌污染物的生长也有重要作用。本研究通过Claisen-Schmidt缩合反应合成了4′-羟基-4-甲氧基查尔酮。用气相色谱-质谱联用(GC-MS)对查尔酮化合物进行了质谱表征,用傅里叶变换红外(FTIR)对官能团进行了鉴定。所用血液制品中的细菌污染物:不动杆菌、大肠杆菌、芽孢杆菌和金黄色葡萄球菌。此外,从美国型培养收集(ATCC)金黄色葡萄球菌被用作比较分离物。采用抑菌面积法对其抑菌活性进行分析。结果表明,该反应产物以黄色晶体形式成功合成了4′-羟基-4-甲氧基查尔酮,产率为72,80%,相对纯度为100%,m/z为254。查尔酮类化合物在6.25 ppm不同浓度下的抑菌活性12.5 ppm和25 ppm对革兰氏阳性菌和革兰氏阴性菌的抑制作用仍然相对较弱,因为达到的抑制能力百分比低于50%。
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