Modification Of Polysulfone For Membrane Bound Enzyme Sensors

Abstract

Polymers used in membrane casting generally lacks suitable functional groups necessary for chemical immobilization of enzymes. In continuation of our work to develop membrane bound enzyme sensors, enzymes were chemically bonded on the modified Polysulfone matrix via a spacer, e.g. glutaraldehyde. 2-amino ethyl polysulfone vias derived from the parent polymer via acylation, oxime formation followed by reduction. a amalyse was covalently bound to the modified polymer in suspension with glutaraldehyde. Activity of the immobilised enzyme was measured and found to be significant. Immobilization of other enzymes by similar route and estimation of their activity/stability are in progress.