Identification of effective primers for varieties of winter rye Secale cereals L.

Abstract

Rye (Secale cereale L.) is important food crop in Russia. The study of the genetic diversity of crops is carried out using molecular markers. To identify effective primers, ISSR (Inter Simple Sequence Repeats), a method for determining DNA polymorphism, was used.The selection of ISSR primers was carried out according to the largest number of polymorphic DNA fragments, their clear amplification and reproducibility in repeated PCR. Of the 22 analyzed ISSR primers with the total DNA of S. cereale, 5 ISSR primers demonstrated high efficiency for the studied 5 winter rye varieties. For further molecular genetic analysis of S. cereale, the following four dinucleotide ISSR primers (M1 (AC)8CG, ISSR-5 (AG)8CA, CR-215 (CT)6TG, CR-218 (GA)6CC) and one trinucleotide ISSR primer X10 (AGC)6C. The identified ISSR primers will make it possible to determine the genetic