Application of combined multiplex-PCR and RT-LAMP to detect Dengue Fever, from clinically infected patients in Surabaya, Indonesia

Abstract

Dengue virus is an important Flavivirus causing Dengue fever, a substantial public health cases in Indonesia. This article reported the application of multiplex PCR and RT-LAMP to detect Dengue virus from suspected infected-patients hospitalized in Dr. Soetomo hospital, Surabaya. In this study both RT-PCR and RT-LAMP could provide a definitive results for less than 2 h of work. Therefore, this experiment showed that the method could be applied for Dengue virus circulating from field samples and be used as routine laboratory testing or further epidemiology study.