Protective Effect of Green Tea Extract on Leptin and Lipid Profile Levels as Result of Induction of Depot Medroxyprogesterone Acetate

Abstract

Objective: The purpose of this study was to investigate the effects of administration of green tea extract in reducing visceral fat, leptin level, and improvement of lipid profile in female rats injected with depot medroxyprogesterone acetate (DMPA). Material and methods: A total of thirty Rattus norvegicus rats were divided into five groups (n=6), consisting of control group (without treatment), group receiving DMPA injection, and group receiving DMPA injection and administration of various doses of green tea extract. Fat tissues were weighed by Ohaus scale. Leptin analysis was done by ELISA technique. Analysis of HDL and LDL levels was performed by spectrophotometry. Results: DMPA induced the increase in leptin level significantly compared with control group (p<0.05). This increase can be reduced by all doses of green tea extract, even reaching a value equal to control group for both highest doses (p>0.05). DMPA significantly increased LDL level compared with control group (p<0.05), and can be restored by control group level by administration of the highest green tea extract dose. DMPA triggered a decrease in HDL level significantly compare to the control group (p<0.05). Management of first green tea extract dose can achieve HDL level equal to control group (p>0.05). Conclusions: It was concluded that green tea extract can protect metabolic status through decreased leptin and improvement of lipid profile induced by DMPA.