Behaviour of polyclonal human IgE in the course of fractionation.

Abstract

Normal human pooled plasma was fractionated by a variety of methods. The IgE concentration of the different fractions was determined by a solid-phase radioimmunoassay. The results of these studies indicate, that polyclonal IgE behaves similarly to IgE of myeloma origin. A biospecific method was worked out to purify IgE from fraction III of the cold ethanol fractionation procedure.