Comparison of antigen-type immunosorbents prepared by different ways.

Abstract

7 types of IgG-containing immunosorbents were compared from different respects by using them for adsorption of antibodies. The human IgG was insolubilized by ethylchlorophormate and glutaraldehyde, thereafter it was bound to activated agarose and polyacrylamide gels and 3 new types of polyacrylamide immunosorbent were prepared by polymerizing the acrylamide in the protein solution and by coupling the protein to the matrix by different ways. Using batch technique, the ethylchlorophormate, the glutaraldehyde and some of the new polyacrylamide immunosorbents proved to be useful, but in almost every respect the immunosorbent prepared from Sepharose gel was the best.