Evaluation of In Vitro Antioxidant and Anti-Lipid Peroxidation Activities of Ching-Pien-Tsao (Pteris multifida Poiret)

Abstract

The total antioxidant capacity (TAC), scavenging ability on 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) and superoxide anions, chelating effect on copper ions, and anti FeCl2/H2O2-stimulated linoleic acid peroxidation of aqueous extract of Pteris multifida Poiret (AEPM), were determined in vitro. Results indicated that the AEPM showed a high TAC (86.7% at 20 mg/mL) with ferric thiocyanate method and exhibited the highest Trolox equivalent antioxidant capacity (1.42 mM at 10mg/mL). The scavenging ability on superoxide anion and chelating effect on copper ions of AEPM were 95.4% and 44.7% at 20mg/mL, respectively. The linoleic acid peroxidation inhibition percentage was increased with increasing concentrations and the IC50 was 1000±42μg/mL.