Type of Cell Separator, Fenwal Amicus Vs COBE Spectra LRS, May Influence the Corpuscular Elements Value of the Donor`s Blood (SN-ICFC Procedure)

Abstract

Background: During the plateletpheresis procedure the number of thrombocytes in the donor’s blood significantly decreases, and the levels of the hematocrit, hemoglobin, and leukocyte diminish as well. Influence of the cell separator is one of the factors that affects the levels of HCT, Hgb and WBC. Objective: In this study, the goal was to determine the value difference of HCT, Hgb, WBC, and platelets after the platelet pheresis process between performance on Fenwal AMICUS and on COBE Spectra LRS Methods: The criteria for participation: male in the age range of 25-45. We have formed two groups: group I–180 separation were performed on 60 participants were the values of hematocrits, concentration of hemoglobin and number of leukocytes ware established before and after separation using the single-needle intermitent flow cell separation (SN-IFCS) on the Fenwal AMICUS device. Group II – presented 180 separation that were performed on 60 participants, were the values of hematocrits, concentration of hemoglobin and number of leukocytes ware established before and after separation using double-needle continuous flow cell separation (SN-IFCS) on the COBE Spectra LRS. device. To confirm the statistical differences we have used Student t-test for independent or dependent samples, as well as Mann-Whitney U test as non-parametric alternative. To compare differences between the values of four parameters (P1-P2) from two groups (using two devices–Fenwal AMICUS and Fresenius Com Tec) we have performed the ANOVA variance analyze. The possibility of errors were accepted for α<0,05, and the difference between groups as statistical relevant were accepted for p<0,05. Results Statistically significant lower values were noted for all researched parameters after separation on both devices (Fenwal AMICUS and COBE Spectra LRS). Comparing the parameters of hematocrit, leukocytes and hemoglobin’s, we found a statistical significant difference in the decrease of the parameter’s value after separation, there was a significant decrease change in using COBE Spectra LRS cell separator compared to using Fenwal AMICUS cell separator with the same procedure. For the platelets (Plt) there was no statistical significant difference (p> 0,05-α=0,05), between average level obtained using either Fenwal AMICUS or COBE Spectra LRS. Conclusion: The type of cell separator had the influence on the decrease value of the observed parameters.