TEST OF ANTIOXIDANT CONTENT IN BLUE LOTUS (Nymphaea Stellata Wild) LEAVES WITH DPPH METHOD (1,1-Diphenyl-2-Picrylhydrazyl)

Jurnal Jamu Kusuma Pub Date : 2021-12-31 DOI:10.37341/jurnaljamukusuma.v1i2.10

Beta Herilla Sekti

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Abstract

Antioxidants are compounds materials that can inhibit the rate of oxidation of other molecules or neutralize free radicals. Antioxidants can be obtained in synthetic or natural forms. Natural antioxidants can protect the human body from damage to active oxygen which can inhibit degenerative diseases and inhibit the production of fat and food. Natural antioxidants can be obtained from plants, one of which is the Blue Lotus Plant (Nymphaea Stellata Wild). To ensure the presence of antioxidants in this plant, the antioxidant activity was tested using the DPPH method. The DPPH method was used to determine the free radical scavenging activity of the Ethanol Extract of Blue Lotus Leaf (Nymphaea Stellata Wild). The positive control used was Ascorbic Acid. The IC50 value obtained for testing the antioxidant content of blue lotus leaf is 78.405 ppm ± 24.607. And Ascorbic Acid 2,238 ppm. It can be concluded that the antioxidant activity of Blue Lotus Leaf is not too different from the positive control of ascorbic acid. The antioxidant activity of blue lotus leaf was stated to be strong and ascorbic acid was stated to be very strong. Keywords: Antioxidant, Free radicals, DPPH

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DPPH法(1,1-二苯基-2-苦酰肼)测定蓝莲花叶中抗氧化剂含量

抗氧化剂是可以抑制其他分子氧化速率或中和自由基的化合物材料。抗氧化剂可以合成或天然形式获得。天然抗氧化剂可以保护人体免受活性氧的损害，可以抑制退行性疾病，抑制脂肪和食物的产生。天然抗氧化剂可以从植物中获得，其中一种是蓝莲花(Nymphaea Stellata Wild)。为了保证该植物中抗氧化剂的存在，采用DPPH法测定了其抗氧化活性。采用DPPH法测定了蓝荷叶乙醇提取物对自由基的清除能力。阳性对照采用抗坏血酸。测定蓝荷叶抗氧化剂含量的IC50值为78.405 ppm±24.607。抗坏血酸2238 ppm。由此可见，蓝荷叶的抗氧化活性与阳性对照抗坏血酸相差不大。据说蓝荷叶的抗氧化活性很强，抗坏血酸很强。关键词:抗氧化剂，自由基，DPPH

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Jurnal Jamu Kusuma

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