Exploring of Anticariogenic Activity of Herbal Formulation from Selected Five Medicinal Plants Leaves

Abstract

Background: Oral diseases continue to be a major health problem worldwide. Plant leaves extracts of formulation have great source as anticariogenic compound against oral pathogenic microorganisms, which can be used to treat infectious diseases. Objective: The leaf and their formulation extracts were tested for their effect on anticariogenic organisms under in vitro condition. Attempt was also made to characterize bioactive compounds at primary level. Material and Methods: The dried plant leaves materials are extracted by cold extraction using hexane, ethyl acetate, methanol, and distilled water. The solvents were evaporated, and the dried masses were suspended in dimethyl sulfoxide and used for anticariogenic activity by agar well diffusion method. 12 different herbal formulations prepared and evaluation their their efficacy against anticariogenic bacteria. Minimum inhibitory concentration (MIC) was evaluated by two-fold serial broth dilution method. Preliminary phytochemical analysis of effective formulation was carried out by thin-layer chromatography (TLC). Results: Plant leaves (Lantana camara var.aculcata (L.) Mold; Manilkara hexandra (Roxb.) Dub; Mangifera indica L; Piper betle L; Syzygium rubicundum Wight & Arn extracts and twelve different formulation prepared by leaves extracts was analysis for their anticariogenic activities against oral microorganisms. All the crude plant leaves extracts and different formulation prepared by leaves extracts exhibited varying degree of growth inhibition in all the oral microorganisms. The activity was determined against, Lactobacillus acidophilus, Lactobacillus casei, Staphylococcus aureus and Streptococcus pyogenes. Maximum zone of inhibition (22 mm) was found when plant leaves extracts of formualtion tested against Lactobacillus casei. Preliminary phytochemical analysis of formulation present the Tannins, Saponins, Steroids and phenolic compound are present.