Molecular Analysis of Rhizopus Fungal Species on Deteriorating Historical Sandstone Monuments: An Advanced Approach

Swati Shakya, N. Tripathi, S. Bhadauria
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Abstract

Sandstone  monuments  may  support  the  broad  categories  of  microorganism  that  are active  in  process  of  biodeterioration. Sandstone monuments have the compound of consortia of Bryophyte and fungi. Fungi  is considered  the most  effective  group  of microorganism causing  biodeterioration  of  sandstone  monuments. Fungal  units  grows in  the  outdoor  conditions  in  presence  of  low  amount  of  nutrition  and  water  it  can start  fungal  colonization and  the  biodeterioration  processes. Observing microorganisms on cultural heritage objects does not automatically assume that they actually change the physical properties or chemical composition of the sandstone. Our research study goals are Seclusion as well as Recognition of Fungi by utilizing molecular methods RFLP as well as RAPD. Eleven  fungal  species  were isolated  having  dominance  over sandstone  structures  of  the  different  monuments. During  the  investigation  it  was  observe  that Aspergillus  species  are  dominant  than Rhizopus  species.  Micro fungi  cause  discoloration  and  mechanical  exfoliation  of different  monuments  that  was  analyzed  production  of  dark  pigments, Hyphae penetration  and  organic  Acid  during  the  identification  using  molecular  technique. Therefore, in this review, we summarise the current knowledge about Rhizopus.
变质砂岩历史遗迹根霉真菌种类分子分析研究进展
砂岩遗迹可能支持在生物退化过程中活跃的多种微生物。砂岩遗迹是苔藓植物和真菌的复合群落。真菌被认为是导致砂岩纪念碑生物退化的最有效的微生物群。真菌单位生长在室外条件下,存在少量的营养和水,它可以开始真菌定植和生物降解过程。观察文化遗产上的微生物并不能自动假设它们实际上改变了砂岩的物理性质或化学成分。我们的研究目标是利用分子方法RFLP和RAPD对真菌进行隔离和识别。11种真菌在不同纪念碑的砂岩结构中具有优势。在调查过程中,观察到曲霉种比根霉种占优势。微真菌引起不同遗迹的变色和机械剥落,在分子鉴定过程中分析了暗色素、菌丝渗透和有机酸的产生。因此,本文对根霉的研究现状进行了综述。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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