Isolation Of Extended-Spectrum B-Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumiae From Dr. Zainoel Abidin General Hospital, Aceh

The International Journal of Tropical Veterinary and Biomedical Research Pub Date : 2019-05-01 DOI:10.21157/IJTVBR.V4I1.13806

Z. Hayati, S. Rizal, Ridhia Putri

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引用次数: 9

Abstract

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum β-lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective

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亚齐省Zainoel Abidin总医院分离产生大肠杆菌和肺炎克雷伯菌的广谱b -内酰胺酶(ESBL

与引起感染的细菌耐多药(MDR)日益增加相比，印度尼西亚发生的感染比以前显著增加。2013年在印度尼西亚5家医院进行的一项研究表明，产生β-内酰胺酶(ESBL)的广谱细菌的患病率达到32-68%。本研究的目的是检测班达亚齐Zainoel Abidin医生总医院产生esbl的大肠杆菌和肺炎克雷伯菌的流行情况和耐药模式。本研究于2016年9月1日至2016年12月31日进行。本研究的标本类型包括血液、痰、尿、脓、粘膜拭子和另一种体液样本。本研究的采样方法为全采样，即全部临床标本在临床微生物实验室检测。采用VITEK-2型生化机(Biomerieux)对产esbl菌进行分离鉴定。本研究共分离出大肠杆菌和肺炎克雷伯菌122株。其中48株(39%)大肠杆菌和74株(61%)肺炎克雷伯菌分离株。从48株大肠杆菌中分离出41株(85%)具有ESBL表型，从74株肺炎克雷伯菌中分离出59株(80%)具有ESBL表型。122株分离株中有100株(82%)具有ESBL表型。产esbls大肠杆菌和肺炎克雷伯菌在27株尿液分离株中分离出24株(89%)，19株血液分离株中分离出18株(95%)，36株痰分离株中分离出28株(78%)，40株脓分离株中分离出30株(75%)。大肠杆菌和肺炎克雷伯菌对amycasin和美罗培南的敏感性较高，均在89%以上。同时对磷霉素和哌环素-他唑巴坦的敏感性分别为80%和77%。另一种抗生素效果较差

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The International Journal of Tropical Veterinary and Biomedical Research

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