The case for colorimetric two-point stopped-enzyme-activity determinations exhibiting zero-order reactions.

Abstract

A careful assessment is made of the relative merits of the two common approaches to enzyme determinations: the so-called kinetic and the two-point colorimetric. A plea is made for a pluralistic approach to the selection of enzyme methods based on a full understanding of the modalities of each procedure. A rational approach to clinical enzymology demands an intellignet choice of methods, irrespective of mode, that will best suit a particular laboratory situation.