Growth performance of Spirulina platensis in supernatant of digested rotten guava

Abstract

This study was performed to evaluate the culture potentiality and growth performance of Spirulina (Spirulina platensis) in supernatant of three different concentrations of digested rotten guava (DRG) and Kosaric Medium (KM) as control. The study consisted of three different concentrations of rotten guavas, such as 20 (T1), 40 (T2) and 60 (T3) were allowed to digest under aeration. After 34 days, three replications showed the reddish white-colored supernatant screened and taken in a 2.0 L conical flask. Then, Spirulina was inoculated to grow in these three digested rotten guava media (DRGM) with the addition of micronutrients and KM and 9.0 g/L NaHCO3 for 14 days. The cell weight of Spirulina was attained at a maximum of 12.43±0.10 mg/L (dry wt. basis) in KM, followed by 0.818 ± 0.002, 0.815 ± 0.0014 and 0.809± 0.0011 mg/L in supernatant of 60, 20 and 40% DRGM, respectively on the 10th day of culture. A similar trend was also observed in the case of specific growth rates (based on cell weight and chlorophyll a) and total biomass (mg/L) of Spirulina. Cell weight of Spirulina had a highly significant (P<0.01) correlation with the chlorophyll a content (r= 0.746) and total biomass (r= 0.742) of Spirulina. The results on the growth performance showed that Spirulina grown in supernatant of 60% DRGM was significantly (P<0.01) higher (T3) than that of Spirulina grown in supernatant of 20% (T1) and 40% DRGM (T2).