Methods for Retrospective Detection of Exposure to Toxic Scheduled Chemicals. Part B: Mass Spectrometric and Immunochemical Analysis of Covalent Adducts to Proteins and DNA

D. Noort, R. Black
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引用次数: 8

Abstract

In this article, an overview of themethods that are currently available for retrospective detection of exposure to a number of chemical warfare agents (CWA), based on adducts formed with macromolecules such as proteins, is presented. These methods can be applied for various purposes, e.g. diagnosis and dosimetry of exposure of casualties, confirmation of nonexposure, verification of nonadherence to the Chemical Weapons Convention, health surveillance, and forensic purposes. The advantage of using protein adducts as biomarkers in comparison with free metabolites is that they are potentially much more longlived. The methods are predominantly based on LC/MS analysis of enzymatic digests of the (modified) proteins or on selective removal of the specific adduct moiety from the protein, followed by GC/MS or LC/MS. Several of the methods have been successfully applied to actual cases and were shown to be highly retrospective.
有毒化学品暴露的回顾性检测方法。B部分:蛋白质和DNA共价加合物的质谱和免疫化学分析
在本文中,概述了目前可用于回顾性检测暴露于一些化学战剂(CWA)的方法,这些方法基于与大分子(如蛋白质)形成的加合物。这些方法可用于各种目的,例如对伤亡人员的接触进行诊断和剂量测定、确认未接触、核查不遵守《化学武器公约》、健康监测和法医目的。与游离代谢物相比,使用蛋白质加合物作为生物标志物的优势在于它们的寿命可能更长。这些方法主要是基于酶解(修饰)蛋白质的LC/MS分析或选择性去除蛋白质中的特定加合物片段,然后是GC/MS或LC/MS。一些方法已成功地应用于实际病例,并被证明是高度回顾性的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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