Application of Mathematical Models and Microfluidics in the Analysis of Saliva Mixing with Antiseptic Solutions

Nina Jelenčiaková, B. Petrović, Sanja P. Kojic, Jovana Jevremov, Stevan Hinić
{"title":"Application of Mathematical Models and Microfluidics in the Analysis of Saliva Mixing with Antiseptic Solutions","authors":"Nina Jelenčiaková, B. Petrović, Sanja P. Kojic, Jovana Jevremov, Stevan Hinić","doi":"10.2478/bjdm-2020-0014","DOIUrl":null,"url":null,"abstract":"Summary Background/Aim: Human saliva offers many advantages over blood-based biochemical assays, therefore, becomes the biological fluid of interest. Once antiseptic solutions react with saliva, both fluids undergo significant changes of their biophysical properties, consequently, those changes have an impact on their principal function. Material and Methods: In this study, saliva was collected and mixed with 0,1% chlorhexidine digluconate solution, fluoride mouthwash, zinc-hydroxyapatite solution and CPP-ACP paste. Microfluidic PVC/Green tape chips within the experimental setup were used to simulate solution mixing. The chip had 2 inlets and 1 outlet, and channel was designed in Y shape without any obstacles. The inlet channels were set at a 60° angle. The channel width was 600 µm and the diameter of inlets and outlet was 2 mm. For better visualization, blue food coloring was added to the saliva. The procedure was recorded with digital USB microscope camera and afterwards the percentage of mixing was obtained by MATLAB programming language. Results: Obtained results show incomplete mixing of all the solutions with saliva. The value of mixed liquid, when mixing 0,1% chlorhexidine digluconate solution with saliva was 51,11%. In case of medium concentration fluoride mouthwash, result was 84,37%. Zinc hydroxyapatite solution obtained result of 85,24%, and the fourth tested solution, CPP-ACP paste, 83,89%. Conclusions: Analyzed mouthwashes exhibit specific, non uniform behavior during mixing with saliva. Microfluidic setups could be efficiently used in simulating real clinical conditions in laboratory settings. Image processing mathematical models are applicable, accurate and useful in determination of the interaction of saliva with commonly used antiseptic solutions.","PeriodicalId":161469,"journal":{"name":"Balkan Journal of Dental Medicine","volume":"304 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Balkan Journal of Dental Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/bjdm-2020-0014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Summary Background/Aim: Human saliva offers many advantages over blood-based biochemical assays, therefore, becomes the biological fluid of interest. Once antiseptic solutions react with saliva, both fluids undergo significant changes of their biophysical properties, consequently, those changes have an impact on their principal function. Material and Methods: In this study, saliva was collected and mixed with 0,1% chlorhexidine digluconate solution, fluoride mouthwash, zinc-hydroxyapatite solution and CPP-ACP paste. Microfluidic PVC/Green tape chips within the experimental setup were used to simulate solution mixing. The chip had 2 inlets and 1 outlet, and channel was designed in Y shape without any obstacles. The inlet channels were set at a 60° angle. The channel width was 600 µm and the diameter of inlets and outlet was 2 mm. For better visualization, blue food coloring was added to the saliva. The procedure was recorded with digital USB microscope camera and afterwards the percentage of mixing was obtained by MATLAB programming language. Results: Obtained results show incomplete mixing of all the solutions with saliva. The value of mixed liquid, when mixing 0,1% chlorhexidine digluconate solution with saliva was 51,11%. In case of medium concentration fluoride mouthwash, result was 84,37%. Zinc hydroxyapatite solution obtained result of 85,24%, and the fourth tested solution, CPP-ACP paste, 83,89%. Conclusions: Analyzed mouthwashes exhibit specific, non uniform behavior during mixing with saliva. Microfluidic setups could be efficiently used in simulating real clinical conditions in laboratory settings. Image processing mathematical models are applicable, accurate and useful in determination of the interaction of saliva with commonly used antiseptic solutions.
数学模型和微流体技术在唾液与防腐液混合分析中的应用
背景/目的:与血液生化分析相比,人类唾液具有许多优点,因此成为人们感兴趣的生物液体。一旦防腐剂溶液与唾液发生反应,这两种液体的生物物理性质都会发生重大变化,因此,这些变化会影响到它们的主要功能。材料与方法:收集唾液,与0.1%二光酸氯己定溶液、含氟漱口水、锌-羟基磷灰石溶液和pcp - acp膏混合。在实验装置中使用微流控PVC/绿带芯片来模拟溶液混合。芯片有2个入口和1个出口,通道设计成无障碍物的Y形。入口通道设置为60°角。通道宽度为600µm,进出口直径为2mm。为了更好地观察,在唾液中加入了蓝色食用色素。用USB数码显微镜相机记录整个过程,然后用MATLAB编程语言计算混合百分比。结果:所得结果显示所有溶液与唾液混合不完全。0.1%二光酸氯己定溶液与唾液混合时,混合液值为51.11%。中浓度含氟漱口水的合格率为84.37%。锌羟基磷灰石溶液得到的结果为85.24%,第四种测试溶液pcp - acp膏体得到的结果为85.89%。结论:分析的漱口水在与唾液混合时表现出特定的,不均匀的行为。微流控装置可以有效地用于模拟实验室环境中的真实临床条件。图像处理数学模型在测定唾液与常用防腐溶液的相互作用方面是适用、准确和有用的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信