Bacterial Isolates and their Antibiotic Susceptibility in Bile of Patients with Gallstone in Al-Hussein Teaching Hospital

Abstract

The bacterial colonization in gallbladder represent important factor in initiation of gallstone and chronic cholecystitis. The aim of the study was to detect the facultative anaerobic bacterial isolates and determination of the most efficient antibiotics in treatment of gallbladder infections. Bile specimens were collected from 62 patients who have suffered from gallstone cultivated on bacteriological media, the diagnosis of isolates and antibiotic susceptibility test for the following antibiotics (CIP,NOR,AK,CN,CTX,CRO,CL,AMC,SXT,AZM and TET) was done by using standard bacteriological techniques. According to results (14.51%) of the examined bile specimens were positive cultures with predominance of E. coli which represent (54.55%) of isolates followed by Enterobacter spp. (18.18%) with high significant differences (P< 0.001), The flouroquinolones (CIP & NOR) were most effective antibiotics followed by aminoglycosides (AK,CN) and CTX under (P< 0.05). Furthermore, the isolates showed high and complete resistance to other antibiotics. In brief, colonization of bacteria may associated with the formation of gallstone where the E. coli is the most frequent bacterial isolates and the flouroquinolones (CIP & NOR) appear to be highly effective against isolated bacteria and may play a role in treatment of infections. Key wards: Gallstone, Bacterial Colonization, Flouroquinolones.