Plant gene targeting and gene replacement: application to crop genetic improvement

Z. Xiaohui, Ye Zhibiao, Zhang Yuyang, Hou Zheng, Li Hanxia
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Abstract

Recent advances and limitations of using zinc finger nuclease to stimulate a high efficiency of homologous recombination, and chimeric oligonucleotides to promote single base replacement in functional genomic research and plant genetic breeding, are systematically reviewed. Approaches to improve gene targeting efficiency through molecular modification of key pathways in plant homologous recombination are also discussed.
植物基因靶向与基因替代:在作物遗传改良中的应用
本文综述了近年来利用锌指核酸酶促进高效同源重组、利用嵌合寡核苷酸促进单碱基替代等技术在功能基因组研究和植物遗传育种中的研究进展和局限性。讨论了通过对植物同源重组关键通路进行分子修饰来提高基因靶向效率的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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