Types and concentrations of cytokinins in the micropropagation of Anthurium maricense

Cecília Moreira Serafim, Arlene Santisteban Campos, Priscila Bezerra dos Santos Melo, A. D. Castro, A. Carvalho
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引用次数: 2

Abstract

Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.
红掌微繁过程中细胞分裂素的类型和浓度
面对对盆栽植物的需求,红掌被视为一个可行的选择。然而,大多数关于获得小苗的研究都是针对黄花蓟马的,而对黄花蓟马的研究还没有报道。因此,本研究的目的是评价四种不同浓度的细胞分裂素对海参节段离体诱导芽的影响。实验设计采用4 × 4因子完全随机化设计,采用4种细胞分裂素(BAP、ZEA、CIN和2iP)和4种浓度(0、2.22、4.44和6.66 μM),共16个处理,5个试管6个重复,使用一个节段。培养物在25±2°C、光周期16 h、光强30 μmolm-2 s-1的生长室内保存60天。在这段时间之后,评估每个节点形成的芽数。在培养基中添加细胞分裂素对海参芽的离体再生有决定性影响。在含有ZEA(3.87)和BAP(3.30)的培养基中(浓度均为6.66 μM),海参的芽形成数最多。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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