{"title":"Molecular Characterization","authors":"Z. Tuzar","doi":"10.1201/9780367813062-4","DOIUrl":null,"url":null,"abstract":"...............................................................................................................................3 Acknowledgements..............................................................................................................4 List of Tables .......................................................................................................................7 List of Figures ......................................................................................................................8 Chapter 1 Gravitropism....................................................................................................9 The Gravitropic Pathway .......................................................................................10 Perception ..................................................................................................10 Auxin Redistribution..................................................................................13 Response ....................................................................................................16 Signal Transduction ...................................................................................17 Research Focus ......................................................................................................22 Bibliography ..........................................................................................................25 Chapter 2 Characterization of the GPS2 Gene ..............................................................31 Materials and Methods.............................................................................................32 Plant Material, Growth Conditions, and DNA Extraction.........................32 Thermal Asymmetric Interlaced Polymerase Chain Reaction ..................32 Cloning of the PCR Product ......................................................................36 Constructs ..................................................................................................38 Agrobacterium Transformation .................................................................43 Floral Dip and Arabidopsis Transformation ..............................................43","PeriodicalId":120222,"journal":{"name":"Lactam-Based Polyamides","volume":"113 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lactam-Based Polyamides","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1201/9780367813062-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
...............................................................................................................................3 Acknowledgements..............................................................................................................4 List of Tables .......................................................................................................................7 List of Figures ......................................................................................................................8 Chapter 1 Gravitropism....................................................................................................9 The Gravitropic Pathway .......................................................................................10 Perception ..................................................................................................10 Auxin Redistribution..................................................................................13 Response ....................................................................................................16 Signal Transduction ...................................................................................17 Research Focus ......................................................................................................22 Bibliography ..........................................................................................................25 Chapter 2 Characterization of the GPS2 Gene ..............................................................31 Materials and Methods.............................................................................................32 Plant Material, Growth Conditions, and DNA Extraction.........................32 Thermal Asymmetric Interlaced Polymerase Chain Reaction ..................32 Cloning of the PCR Product ......................................................................36 Constructs ..................................................................................................38 Agrobacterium Transformation .................................................................43 Floral Dip and Arabidopsis Transformation ..............................................43