Analysis of the Cytokine Balance in Tear Fluid of Diabetic Patients with Bacterial Keratitis Depending on the Severity of the Disease

O. Zavoloka, P. Bezditko, L. P. Abramova, V. O. Vekshyn
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Abstract

The aim. To analyze the cytokine balance of tear fluid in patients with bacterial keratitis at presentation depending on the severity of the disease and the presence of diabetes mellitus (DM). Materials and methods. The analysis was performed through the comparison of the level of pro- and anti-inflammatory cytokines in the tear fluid of 17 patients with type 1 DM and bacterial keratitis and 15 patients with bacterial keratitis without DM at presentation. Data from 14 healthy individuals of the appropriate age were also used for comparison. The patients with bacterial keratitis were divided into subgroups according to the severity of bacterial keratitis. The levels of IL-1β, IL-6 and IL-10 in the tear fluid of the sick and the contralateral eye were determined by quantitative colorimetric enzyme-linked immunosorbent assay. Results and discussion. At presentation, patients with bacterial keratitis, both with and without DM, showed increased levels of proinflammatory cytokines, namely IL-1β and IL-6, in the tear fluid of the sick eye, which correlated with the severity of the disease, and also increased level of anti-inflammatory cytokine IL-10 in the tear fluid of the contralateral eye. In addition, the concentrations of proinflammatory cytokines IL-1β, IL-6 in the tear fluid of the contralateral eye in DM patients were increased at all degrees of severity of bacterial keratitis. Conclusions. In patients with bacterial keratitis, the cytokine balance of the tear fluid of the sick and the contralateral eye depends on the severity of the disease and the presence of DM. Keywords: diabetes mellitus, bacterial keratitis, severity of keratitis, cytokines, interleukins.
糖尿病合并细菌性角膜炎患者泪液中细胞因子平衡与病情严重程度的关系分析
的目标。分析细菌性角膜炎患者的泪液细胞因子平衡,这取决于疾病的严重程度和糖尿病(DM)的存在。材料和方法。通过比较17例1型糖尿病合并细菌性角膜炎患者和15例无糖尿病的细菌性角膜炎患者泪液中促炎性和抗炎性细胞因子的水平进行分析。来自14名适龄健康个体的数据也被用于比较。根据细菌性角膜炎的严重程度将患者分为亚组。采用定量比色法酶联免疫吸附法测定患者和对侧眼泪液中IL-1β、IL-6、IL-10水平。结果和讨论。在就诊时,细菌性角膜炎患者,无论是否患有糖尿病,均显示患病眼泪液中促炎细胞因子IL-1β和IL-6水平升高,这与疾病的严重程度相关,并且对侧眼泪液中抗炎细胞因子IL-10水平升高。此外,糖尿病患者对侧眼泪液中促炎因子IL-1β、IL-6浓度在不同严重程度的细菌性角膜炎中均升高。结论。在细菌性角膜炎患者中,患者和对侧眼泪液的细胞因子平衡取决于疾病的严重程度和是否存在糖尿病。关键词:糖尿病,细菌性角膜炎,角膜炎的严重程度,细胞因子,白细胞介素。
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