[Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells].

Abstract

PURPOSE To investigation the effect of flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells. METHODS After evaluation of the toxic effect of chrysin, preosteoblast MC3T3-E1 cell suspension was prepared with DMEM and then the cells were cultured with 0 and 25 μmol/L chrysin. Real-time quantitative PCR was used to detect the expression of osteogenic marlcers including Runx2, ColA1 and OCN. Statistical analysis was performed using SPSS17.0 software package. RESULTS 25 μmol/L chrysin had no toxic effect on preosteoblast MC3T3-E1 cells. After co-culture with 25 μmol/L chrysin,the expression of Runx2, ColA1 and OCN was highest at the 7th,14th and 21th day,respectively. CONCLUSIONS 25 μmol/L chrysin can promote osteogenic gene expression of preosteoblast MC3T3-E1 cell.