Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip

Abstract

We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.