Image analysis system for 96-well plate fluorescence assays

Abstract

Fluorescence Assays in 96-well format are becoming routine in laboratory and clinical procedures. So there is a necessity for developing a method capable of high throughput analysis at affordable cost for manufacturing of less expensive readers. This paper focuses on an image based analysis system to for a fluorescent transport liquid assay performed in a 96-well microtiter plate which is acquired as an image by Digital Camera. The Acquired Image is passed on to a developed Image Analysis System incorporating the Neural Network Architecture which categorizes the result as Fast Efflux, Medium Efflux and Slow Efflux.