Isolation and Purification of Nucleic Acids

James J. Greene
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引用次数: 11

Abstract

Ausubel, F. M. et al., “Short Protocols in Molecular Biology”, Third Edition, John Wiley & Sons, Inc., p. 9-52 (1997).* “Extraction and Puri?cation of Plasmid DNA,” Plasmid Vectors, 1.21-1.45. Weith et al., “Synthesis of Cellulose Derivatives Containing the Dihydroxyboryl Group and a Study of Their Capacity to Form Speci?c Complexes with Sugars and Nucleic Acid Components,” Biochemistry, 914396-4401 (1970). Shih et al., “Chemical Linkage of Nucleic Acids to Neutral and Phosphorylated Cellulose Powders and Isolation of Speci?c Sequences by A?inity Chromatography,” Biochemistry, 1313411 3418 (1974). Astell et al., “Thermal Elution of Complementary Sequences of Nucleic Acids from Cellulose Columns with Covalently Attached Oligonucleotides of Known Length and Sequence,” J. Biol. Chem., 24811944-1946 (1971). Kothari et al., “RNA Fractionation on Modi?ed Celluloses,” J. Chromatogr, 731449-462 (1972). Su et al., “Cellulose as a Matrix for Nucleic Acid Puri?cation,” Analytical Biochemistry, 2671415-418 (1999). “Protocol for Genomic DNA Preparation from Fresh or Frozen Serum for PCR Ampli?cation,” BioTechniques, 291460-466 (2000). Ahn et al., “Rapid Mini-Scale Plasmid Isolation for DNA Sequenc ing and Restriction Mapping,” BioTechniques, 291466-468 (2000). Scott et al., “The use of biomagnetic separation to recover DNA suitable for PCR for Claviceps species,” Letters in Applied Microbiology, 31195-99 (2000). Taylor, et al., “Application for magnetite and silica-magnetite composites to the isolation of genomic DNA,” Journal of Chroma tography A, 8901159-166 (2000). Levison et al., “Recent developments of magnetic beads for use in nucleic acid puri?cation,” Journal of Chromatography A, 8161107 111 (1998). Mrazek et al., “Processing of mRNA from Human Leukocytes By Biomagnetical Separation: Comparison with Current Methods of RNA Isolation,”Acta Univ. Palacki. Olomuc., Fac. Med., 142123-28 (1999). Davies et al., “Isolation of Plasmid DNA Using Magnetite as a Solid-Phase Adsorbent,” Analytical Biochemistry, 262192-94 (1998). Kotsopoulos et al., “Isolation of 3.5-kb Fragments on Magnetic Solid Supports,” BioTechniques, 201198-200 (1996). Rudi et al., “Rapid, Universal Method to Isolate PCR-Ready DNA Using Magnetic Beads,” BioTechniques, 221506-511 (1997).
核酸的分离纯化
奥苏贝尔,F. M.等人,“分子生物学的简短协议”,第三版,约翰威利父子公司,第9-52页(1997)。*“提取和纯化?”质粒DNA阳离子,《质粒载体》,1.21-1.45。wewith等人,“含二羟基硼基纤维素衍生物的合成及其形成特异性的能力研究”。[3]生物化学学报,2006(1):1 -4。Shih et al.,“核酸与中性和磷酸化纤维素粉末的化学连锁和种?A的c序列?化学,13134113418(1974)。Astell等人,“用已知长度和序列的共价连接寡核苷酸的纤维素柱热洗脱核酸互补序列”,《J. Biol》。化学。, 24811944-1946(1971)。Kothari等人,“RNA在Modi上的分离?[j] .生物质化学工程,2004(1)。苏等人,“纤维素作为核酸纯化的基质?”《分析生物化学》,2671415-418(1999)。从新鲜或冷冻血清中制备基因组DNA用于PCR扩增的方案?生物工程学报,2004(2)。Ahn et al.,“用于DNA测序和限制性基因图谱的快速小型质粒分离”,生物技术,291466-468(2000)。Scott et al.,“利用生物磁分离技术恢复锁骨虫物种的DNA,”《应用微生物学快报》,31195-99(2000)。Taylor等人,“磁铁矿和二氧化硅-磁铁矿复合材料在基因组DNA分离中的应用”,《Journal of Chroma tography A》,8901159-166(2000)。Levison等人,“用于核酸纯化的磁珠的最新进展?色谱学报A, 8161107111(1998)。Mrazek等人,“生物磁分离处理人类白细胞mRNA:与当前RNA分离方法的比较”,《Palacki大学学报》。Olomuc。前沿空中管制官。医学杂志,142123-28(1999)。Davies等人,“使用磁铁矿作为固相吸附剂分离质粒DNA”,《分析生物化学》,262192-94(1998)。Kotsopoulos等人,“磁性固体支架上3.5 kb片段的分离”,生物技术,201198-200(1996)。Rudi等人,“使用磁珠快速、通用的方法分离PCR-Ready DNA”,生物技术,221506-511(1997)。
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