The occurrence of drug-resistant bacteria and screening the possible presence of residual antibiotics in poultry feed samples

Stamford Journal of Microbiology Pub Date : 2020-12-13 DOI:10.3329/sjm.v10i1.50730

M. Hossain, S. R. Shewly, Chayanika Mazumder, Shah Murshid Uj Jaman Arowan, S. K. Munshi

{"title":"The occurrence of drug-resistant bacteria and screening the possible presence of residual antibiotics in poultry feed samples","authors":"M. Hossain, S. R. Shewly, Chayanika Mazumder, Shah Murshid Uj Jaman Arowan, S. K. Munshi","doi":"10.3329/sjm.v10i1.50730","DOIUrl":null,"url":null,"abstract":"The use of antibiotics in the poultry and livestock industries for the treatment and prevention of infectious diseases, and as growth promoters in poultry feeds has increased worldwide. Such frequent employment of antibiotics may contribute to the development and dissemination of bacterial antibiotic resistance. The present study was an attempt to isolate drug-resistant bacteria and to screen the probability of having residual antibiotics in the poultry feed samples. Therefore, a total of 18 samples inclusive of starter, grower and finisher of two poultry feed brands of reputed Bangladeshi feed companies were collected and subjected to microbiological analysis, antibiogram and agar well diffusion assay. All the samples contained extended numbers of total viable bacteria and fungi in an average of 108 and 107 cfu/g, respectively. Klebsiellaspp., Pseudomonas spp. and Bacillus spp. were predominantly present in the tested samples. E. coli and Vibrio spp. were also found in most of the samples. Most isolates have been determined to be multidrug-resistant. All the isolates showed resistance against Cefuroxime. Penicillin resistance was found in most of the isolates in greater proportion. Higher rate of resistance was evident against Novobiocin, Cephradine and Rifampicin. However, the bacterial isolates showed sensitivity to Tobramycin, Nalidixic acid and Neomycin. The poultry feed samples, especially starter and finisher of both brands noticeably had significant antimicrobial activity against the laboratory isolates indicative of the probable presence of residual antibiotics which might be used as supplements in the poultry feed samples. \nStamford Journal of Microbiology, Vol.10 (1) 2020: 30-34","PeriodicalId":170445,"journal":{"name":"Stamford Journal of Microbiology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stamford Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3329/sjm.v10i1.50730","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 4

Abstract

The use of antibiotics in the poultry and livestock industries for the treatment and prevention of infectious diseases, and as growth promoters in poultry feeds has increased worldwide. Such frequent employment of antibiotics may contribute to the development and dissemination of bacterial antibiotic resistance. The present study was an attempt to isolate drug-resistant bacteria and to screen the probability of having residual antibiotics in the poultry feed samples. Therefore, a total of 18 samples inclusive of starter, grower and finisher of two poultry feed brands of reputed Bangladeshi feed companies were collected and subjected to microbiological analysis, antibiogram and agar well diffusion assay. All the samples contained extended numbers of total viable bacteria and fungi in an average of 108 and 107 cfu/g, respectively. Klebsiellaspp., Pseudomonas spp. and Bacillus spp. were predominantly present in the tested samples. E. coli and Vibrio spp. were also found in most of the samples. Most isolates have been determined to be multidrug-resistant. All the isolates showed resistance against Cefuroxime. Penicillin resistance was found in most of the isolates in greater proportion. Higher rate of resistance was evident against Novobiocin, Cephradine and Rifampicin. However, the bacterial isolates showed sensitivity to Tobramycin, Nalidixic acid and Neomycin. The poultry feed samples, especially starter and finisher of both brands noticeably had significant antimicrobial activity against the laboratory isolates indicative of the probable presence of residual antibiotics which might be used as supplements in the poultry feed samples. Stamford Journal of Microbiology, Vol.10 (1) 2020: 30-34

查看原文本刊更多论文

耐药菌的发生及家禽饲料样品中可能存在的残留抗生素的筛选

在家禽和畜牧业中，抗生素用于治疗和预防传染病，并作为家禽饲料中的生长促进剂在世界范围内有所增加。这种频繁使用抗生素可能会导致细菌抗生素耐药性的发展和传播。本研究旨在分离耐药菌，筛选家禽饲料样品中残留抗生素的可能性。因此，共收集了18个样品，包括孟加拉国知名饲料公司的两个家禽饲料品牌的发酵剂、生长剂和育成剂，并进行了微生物分析、抗生素谱和琼脂孔扩散试验。所有样品的活菌总数和真菌总数平均分别为108和107 cfu/g。Klebsiellaspp。，假单胞菌和芽孢杆菌在检测样品中主要存在。在大部分样本中也发现了大肠杆菌和弧菌。大多数分离株已被确定为多重耐药。所有菌株对头孢呋辛均有耐药性。大多数分离株中均有较大比例的青霉素耐药。新生物素、头孢定和利福平的耐药率较高。然而，分离的细菌对妥布霉素、钠利地酸和新霉素敏感。家禽饲料样品，特别是两种品牌的发酵剂和育尾剂，对实验室分离物具有显著的抗菌活性，表明可能存在残留的抗生素，这些抗生素可能用作家禽饲料样品中的补充剂。微生物学报，Vol.10 (1) 2020: 30-34

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Stamford Journal of Microbiology

自引率

0.00%

发文量