The blood cell counting and classification from stationary suspensions by laser light scattering method

Proceedings of the 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Vol.20 Biomedical Engineering Towards the Year 2000 and Beyond (Cat. No.98CH36286) Pub Date : 1998-10-29 DOI:10.1109/IEMBS.1998.746964

Ye Yang, Zhen-xi Zhang, Xinhui Yang, D. Jiang

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引用次数: 1

Abstract

In order to overcome the disadvantages caused by the flow principle used in automatic counting and classification of blood cells, a new method of counting and classification of blood cells is presented in this paper. This method is a laser light scattering method from stationary suspensions. The blood cell is simulated by an isotropic sphere, and Mie scattering theory is used to describe the scattering of blood cells. In order to get the size distribution of blood cells from their scattered light intensity distribution, the NNLS method combined with Powell method and precision punishment method are used. The dependence between blood cell concentration and its scattered light intensity is obtained from experiment. It is shown that there exists a linear relationship between them. By this new method, the size distribution of red blood cells and white blood cells are obtained, and the white blood cells can be classified as lymphocyte, middle-sized cells and neutrocyte.

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用激光散射法对固定悬液进行血细胞计数和分类

为了克服血液细胞自动计数和分类采用流动原理的缺点，本文提出了一种新的血细胞计数和分类方法。该方法是一种固定悬浮液的激光散射方法。用各向同性球体模拟血细胞，用Mie散射理论描述血细胞的散射。为了从血细胞散射光强分布中得到其大小分布，采用了NNLS法结合Powell法和精密惩罚法。通过实验得到了血细胞浓度与其散射光强度之间的关系。结果表明，两者之间存在线性关系。通过这种新方法，获得了红细胞和白细胞的大小分布，白细胞可分为淋巴细胞、中等大小细胞和中性细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Proceedings of the 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Vol.20 Biomedical Engineering Towards the Year 2000 and Beyond (Cat. No.98CH36286)

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