Comparative effects of garlic (Allium sativum) powder and atorvastatin in female reproductive system of hypercholesterolemic rats: A histological and biochemical evaluation

Journal of Shahrekord University of Medical Sciences Pub Date : 2021-09-29 DOI:10.34172/jsums.2021.22

Sima Jafari, F. Farokhi, A. Sadeghi

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引用次数: 2

Abstract

Background and aims: The abnormal increase in blood cholesterol can cause many problems. Statins have a cholesterol-lowering effect, but they also have adverse effects. Garlic prevents the formation of cholesterol due to its antibiotic properties. This study aimed to investigate the comparative effect of garlic powder and atorvastatin on hypercholesterolemia-induced reproductive failure in female rats. Methods: In the present experimental study, 48 adult female Wistar rats were divided into eight groups (n=6), including control, atorvastatin (10 mg/kg/d; orally), atorvastatin (20 mg/kg/d; orally), garlic powder (100 mg/kg/d; orally), hypercholesterolemia (1.5 mg/kg/d of cholesterol; orally), hypercholesterolemia + atorvastatin (10 mg/kg/d), hypercholesterolemia + atorvastatin (20 mg/kg/d), and hypercholesterolemia + garlic powder. After 30 days, rats were euthanized and blood samples were obtained from their heart for serological assessments. The right ovary was transferred to 10% formalin for histological analyses, and the left ovary was transferred to a −80°C freezer for evaluation of oxidative stress markers. Data were statistically analyzed by ANOVA and Tukey’s test using SPSS version 24.0 (P<0.05). Results: The number of healthy primordial, primary, secondary, and antral follicles, catalase activity, total antioxidant capacity (TAOC) as well as estrogen and progesterone levels were lower in hypercholesterolemic rats compared to controls (P<0.001). Additionally, the number of the atretic primary, secondary, and antral follicles and malondialdehyde (MDA) levels were higher in hypercholesterolemic rats (P<0.001). However, garlic powder and atorvastatin 10 improved alterations in the mentioned parameters (P=0.99). Conclusion: The results showed that hypercholesterolemia could have adverse effects on rat ovaries. However, the garlic powder improves ovarian toxicity in hypercholesterolemia rats better than atorvastatin.

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大蒜粉和阿托伐他汀对高胆固醇血症大鼠雌性生殖系统的比较作用:组织学和生化评价

背景与目的:血胆固醇异常升高可引起许多问题。他汀类药物有降低胆固醇的作用，但也有副作用。由于大蒜的抗生素特性，它可以防止胆固醇的形成。本研究旨在探讨大蒜粉和阿托伐他汀对高胆固醇血症致雌性大鼠生殖功能衰竭的影响。方法:选用成年雌性Wistar大鼠48只，随机分为8组(n=6)，对照组、阿托伐他汀(10 mg/kg/d;口服)，阿托伐他汀(20mg /kg/d;口服)、大蒜粉(100mg /kg/d;口服)，高胆固醇血症(1.5 mg/kg/d胆固醇;口服)、高胆固醇血症+阿托伐他汀(10mg /kg/d)、高胆固醇血症+阿托伐他汀(20mg /kg/d)、高胆固醇血症+大蒜粉。30天后，对大鼠实施安乐死，并从其心脏提取血液样本进行血清学评估。将右卵巢转移到10%福尔马林中进行组织学分析，将左卵巢转移到−80°C的冰箱中评估氧化应激标志物。数据采用方差分析和Tukey检验，采用SPSS 24.0版本进行统计学分析(P<0.05)。结果:与对照组相比，高胆固醇血症大鼠的健康原始、初级、次级和窦泡数量、过氧化氢酶活性、总抗氧化能力(TAOC)以及雌激素和孕酮水平均较低(P<0.001)。此外，高胆固醇血症大鼠的原发性、继发性和窦性卵泡闭锁数量和丙二醛(MDA)水平较高(P<0.001)。然而，大蒜粉和阿托伐他汀10改善了上述参数的改变(P=0.99)。结论:高胆固醇血症对大鼠卵巢有不良影响。然而，大蒜粉比阿托伐他汀更能改善高胆固醇血症大鼠的卵巢毒性。

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Journal of Shahrekord University of Medical Sciences

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