Rapid identification analysis of chemical constituents of Sedum villosum L. (Orpin.) by UHPLC-DAD-HRSM

Abstract

In the present paper, we are interested, for the first time, to separate and identify two glycosyl the flavones from Sedum villosum L, family Crassulaceae collected in the flowering phase in Lakhdar Oued, village of Tlemcen (Algeria). The crude methanolic extract of the flowers part was fractionated on column chromatography, and eluted with dichloromethane/methanol each time with increasing polarity of methanol; 24 fractions were separated. One of these fractions named F16 showed more antioxidant activity to scavenge DPPH free radical with percentage inhibition of 94.849 %. F16 was separated by thin-layer chromatography (TLC) to give 10 compounds. We were chosen the sub-fractions F16.8, which has antioxidative activity of 77.02 %, provided two major molecules of glycosyl the flavones, analysed by ultra-highperformance liquid chromatography coupled to mass spectrometry (UHPLC-DAD-HRSM). Compound 1 was identified as 7, 3'-dihydroxyflavone-5-O-dihexosyl-4'-O-deoxyhexose and compound 2 was 7, 3'-dihydroxyflavone-5-O-hexose- 4'-O-deoxyhexose.