INVESTIGATION OF NEUROPROTECTIVE EFFECT OF SAPINDUS MUKROOSSI EXTRACT ON TYPE1 DIABETE INDUCED NEUROPATHIC PAIN PERCEPTION IN RAT

Abstract

Diabetic Neuropathy is a most common Microvascular diabetic complication, associated with neuropathic disorder causing nerve injury it mainly depends upon duration of hyperglycemia, elevation in glycosylated haemoglobin. Good Neuropathic pain perception can improve Neuropathy. Single intraperitoneal injection of STZ (60 mgkg) was administered to the albino wistar rats induced Diabetes, it showed marked hyperglycemia. Hyperglycemia effect Glycated haemoglobin level in blood and reduce and reduce thermal hyperalgesia (Eddy’s Hot plate, Tail flick), motorcoordination, as compared to the animals of control group. Effect of test drug Sapindus Mukroosi was assessed by behavioural and biochemical parameters (Serum Glucose Level, Lipid peroxidation, reduced Gluthathione) in the brain tissue of wistar rats. After 28 days treatment of Sapindus Mukroosi with low (250 mgkg) and higher (500 mgkg) dose in diabetic rats showed antidiabetic action by reduction in hyperglycemia. Sapindus showed good result also in behavioural and biochemical parameters such as Thermal hyperalgesia, motor coordination in comparison with diabetic control group. The most promising effect of Sapindus seen with 500mgkg after treating period. It significantly decrease serum glucose level, Glycated haemoglobin, lipid peroxidation and improving reduced gluthathione level as compared to diabetic control rats. Thus Sapindus Mukroosi due to its antioxidant and antidiabetic property can be concluded as a effective or preventive treatment for Diabetic Neuropathy. Streptozotocin induce hyperglycemia and causes increase in oxidative stress and this conditions are oppose by treatment with Sapindus Mukroosi by inhibit the generation of free radicals and improving self antioxidant GSH level significantly.