[Chromatographic analysis of water-soluble vitamins. I- Chromatographic behaviour of water-soluble vitamins on Aminex resins (author's transl)].

Abstract

The most important water-soluble vitamins are quantitatively determined by high pressure chromatography using porous ion exchange resins. Thiamine and Riboflavine are separated by a cation exchange resin Aminex A5 using 0.3 M KCI in 0.1 M potassium phosphate pH 8.0 buffer as mobile phase; pyridoxamine, pyridoxol, pirydoxal and nicotinamide are successfully chromatographated on the same type of cationic resin using a linear gradient both of pH and ionic strength as mobile phase. Anionic vitamins like L-Ascorbic Acid and Nicotinic Acid are separated on Aminex A14 at acidic values of pH, using isocratic conditions of the ionic modifier. Under the described experimental conditions, the reproducibility of VR, RS and peak area are of 2.5% and 3% respectively. The sensitivity relative to the vitamins tested ranges from 100 to 250 ng.