Use of silicon and GaAs pixel detectors for digital autoradiography

1996 IEEE Nuclear Science Symposium. Conference Record Pub Date : 1996-12-31 DOI:10.1109/NSSMIC.1996.591467

E. Bertolucci, M. Conti, G. Grossi, E. Mancini, P. Russo, M. Campbell, E. Chesi, E. Heijne, P. Middelkamp, L. Scharfetter, P. Randaccio, R. Beccherle, A. Del Guerra, M. Gambaccini, R. Marchesini, C. Da Via, S. D'Auria, V. O’Shea, K. Smith, W. Snoeys, S.R. Amedolia, M. G. Bisogni, U. Bottigli, M. Ciocci, M. Fantacci, N. Romeo, V. Rosso, O.A. Stefanini

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引用次数: 25

Abstract

Solid state detectors made of Si (4.8/spl times/8 mm/sup 2/) and GaAs (6.4/spl times/8 mm/sup 2/) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with /sup 32/P. The system has shown a good minimum detectable activity per unit area of 2.10/sup -4/ cps mm/sup -2/, and has proved linear for count rate in the range 0.2-20 cps, typical of autoradiography. The pixel dimensions are 75/spl times/500 /spl mu/m/sup 2/ (Si-Omega2) and 50/spl times/500 /spl mu/m/sup 2/(GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).

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使用硅和砷化镓像素探测器的数字自放射照相

由Si (4.8/spl倍/ 8mm /sup 2/)和GaAs (6.4/spl倍/ 8mm /sup 2/)像素矩阵与欧洲核子研究中心为hp实验开发的Omega2和Omega3电子读出系统碰撞结合制成的固态探测器，已用于获得通过电泳分离的人类上皮细胞群和DNA片段的放射自显像，均标记为/sup 32/P。该系统显示出良好的单位面积最小可检测活度，为2.10/sup -4/ cps mm/sup -2/，并且在0.2-20 cps范围内的计数率呈线性，这是典型的放射自显影。像素尺寸分别为75/spl倍/500 /spl mu/m/sup 2/(Si-Omega2)和50/spl倍/500 /spl mu/m/sup 2/(GaAs-Omega3)。我们能够在纸上的电泳凝胶(斑点)上清楚地定位含有放射性示踪剂和DNA片段的细胞簇。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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1996 IEEE Nuclear Science Symposium. Conference Record

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