The Anti-Hyperplasia of Mammary Gland Effect of Chinese Traditional Medicine compound RPXT in Rats

Abstract

Rupixiao Tablets (RPXT) is a Chinese Traditional Medicine compound and comprises of 15 Chinese medicines, but the effect of anti-mammary gland hyperplasia, toxicity and mechanism are not clear. We investigated the protective effect of RPXT on hyperplasia of mammary gland (HMG) induced by estrogen and progestogen in SD rats. The rats were randomly divided into three groups (n = 10), including normal control group, HMG model group and RPXT treatment group. The toxicity study of RPXT on HMG rats was performed at 500 mg/kg/day by i.g. for 35 consecutive days. Changes of nipple height and diameter, serum sex hormones levels and organ indexes, pathologic changes of mammary gland, heart, liver, spleen, kidney and lung, hematological and biochemical analysis were measured or observed to evaluate the anti-HMG effect and toxicology of RPXT. The expression of AP-2α, P53 and caspase-3 in mammary gland of rats were analyzed by western blot. The results indicated that RPXT could remarkably decrease nipple height and diameter, uterus index and E2 concentration of HMG rat serum, and no toxicity at dose of 500 mg/ kg/day. AP-2α, P53 and caspase-3 expression in mammary gland was significantly decreased in mammary gland of RPXT -treated HMG rats. The whole results indicated that RPXT has protective and therapeutic effects on HMG rats, and is a promising Chinese Traditional Medicine for human mammary cancer prevention which is related to caspase3, AP-2α and P53 expression.