Methode zum direkten nachweis von Escherichia coli in wasser

Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1981-06-01 DOI:10.1016/S0721-9571(81)80018-X

Theo Burki, Titus Emmenegger, Werner Ettel , Urs Fröhlicher, Hans Illi, Fritz Marti, Walter Stutz

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Abstract

A rapid membrane filter method employing ECD agar for the direct enumeration of Escherichia coli type 1 in water is described. After a preincubation period of 4 h at 37 °C on trypticase soy agar the membrane is transferred to ECD agar for an additional 12–24 h incubation at 44 °C. E. coli colonies are identified by a positive indole test performed directly on the membrane.

E. coli colonies are easily recognized within a few minutes by their red color and a red halo surrounding the colony. The positively evaluated colonies from natural samples were confirmed to indeed be comprised of E. coli to an extent of 98%.

No difference in E. coli recovery from natural samples was obtained by these means when compared with tests utilizing either M-FC medium or tergitol 7 agar. Regarding specificity, however, the described method was found to be superior to both the M-FC and the tergitol 7 procedures.

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圣城别人的行为

介绍了一种利用ECD琼脂快速膜过滤法直接枚举水中1型大肠杆菌的方法。在胰酶大豆琼脂上37°C预孵育4小时后，将膜转移到ECD琼脂上，在44°C下再孵育12-24小时。大肠杆菌菌落是通过直接在膜上进行阳性吲哚试验来鉴定的。大肠杆菌菌落在几分钟内就很容易被识别出来，因为它们的红色和周围的红色光环。从天然样品中得到的阳性菌落被证实确实由大肠杆菌组成，其程度达到98%。与使用M-FC培养基或tergitol 7琼脂进行的测试相比，通过这些方法从天然样品中获得的大肠杆菌回收率没有差异。然而，就特异性而言，所描述的方法被发现优于M-FC和tergitol 7程序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie

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