TOKSISITAS EKSTRAK ETIL ASETAT KULIT BIJI JENGKOL

Talenta Conference Series: Science and Technology (ST) Pub Date : 2019-06-30 DOI:10.32734/st.v2i2.497

Misri Yanti Lubis, Lamek Marpaung, M. P. Nasution, Partomuan Simanjuntak

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Abstract

Penelitian ini melakukan kajian terhadap toksisitas ekstrak etil asetat dari kuli biji jengkol (Archidendron jiringa) Jack. I. C. Nielsen) dengan menggunakan metode Brine Shrimp Lethality Test (BSLT). Nilai LC50 ekstrak diperoleh dari persamaan regresi linear dari log konsentrasi vs probit % mortalitas. Kulit biji jengkol dikeringkan pada temperature ruang selama 1 x 24 jam dan dimaserasi dengan methanol. Filtrat yang diperoleh diuapkan dengan menggunakan rotary evaporator untuk memperoleh ekstrak pekat methanol. Selanjutnya, ekstrak pekat methanol dilarutkan dalam air dan dipartisi dengan etil asetat, diulangi beberapa kali, dan diuapkan kembali untuk memperoleh ekstrak pekat etil asetat. Uji fitokimia untuk penentuan kandung fenolik dilakukan dengan FeCl3. Uji kandungan fenol dengan FeCl3 menunjukkan hasil positif untuk ekstrak etil asetat. Nilai LC50 untuk ekstrak etil asetat adalah 14.19 ppm. Berdasarkan nilai LC50 ekstrak etil asetat kulit biji jengkol dikategorikan sangat toksik. Toksisitas ekstrak etil asetat dapat diasosiasikan oleh kandungan senyawa fenol dalam ekstrak. This research studied about toxicity ethyl acetate extract of jiringa (Archidendron jiringa) Jack. I. C. Nielsen) pods by using Brine Shrimp Letality Test (BSLT) method. The LC50 of extract obtained from linear regression equation on chart log concentration vs probit % mortality. Pods of jiringa dried at room temperature 1 x 24 h and then macerated with methanol. Filtrat were evaporated with rotary evapoarator to obtained methanol extract. Further, methanol extract dissolved with water and partitioned with ethyl acetate for several times, and then evaporated to obtained ethyl acetate extract. Test consitution of phenolic compound performed by using FeCl3. Test phenolic compounds with FeCl3 showed positive test for ethyl acetate. The LC50 values of ethyl acetate was 14.19 ppm. Ethyl acetate extract of jiringa (Archidendron jiringa) Jack. I. C. Nielsen) pods showed high toxicity. The high toxicity cause of phenolic compounds constitution. Ethyl acetate extract of jiringa’s pods has high toxicity because it contains phenolic compounds.

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芦荟提取物的毒性

这项研究做苦力的醋酸纤维素乙醇提取物毒性研究种子豪厄尔(Archidendron jiringa)杰克。I . C . Nielsen)用盐水虾的致死率测试方法(BSLT)。获得LC50值提取日志vs probit %浓度的线性回归方程的死亡率。豪厄尔种子干燥的皮肤在空间的温度和methanol dimaserasi 1×24小时。diuapkan用获得的蒸发器获得扶轮滤液浓缩提取物methanol。接下来,浓缩提取物methanol溶解在水中和醋酸纤维素乙醇,重复几次,运行diuapkan又获得浓醋酸纤维素乙醇提取物。植物化学试验用FeCl3酚做真正的测定。不是用FeCl3含量测试显示:醋酸纤维素乙醇提取物的积极结果。醋酸纤维素乙醇提取物的LC50值是19 mtc 14。根据皮肤LC50值醋酸纤维素乙醇提取物豪厄尔被剧毒的种子。毒性:醋酸纤维素乙醇提取物可以被提取物中的不是化合物含量相关联。这个研究studied jiringa之关于醋酸toxicity乙基extract (Archidendron jiringa)杰克。I . C . Nielsen)豆荚:用盐水虾Letality测试(BSLT)方法。extract LC50》获得从线性regression equation图表上的双臀vs probit %不朽日志。豆荚的jiringa dried at房间温度1×24 h然后macerated methanol同在。是evaporated滤液与evapoarator为了获得扶轮methanol extract。更远,methanol extract dissolved水和醋酸和乙基partitioned好几个时报,然后evaporated来说获得乙基醋酸extract。酚化合物用FeCl3 performed by之consitution测试。测试用FeCl3酚compounds那里积极测试醋酸为乙基。乙基之价值观的LC50 19 mtc醋酸是14。乙基jiringa的醋酸extract (Archidendron jiringa)杰克。I . C .尼尔森toxicity)豆荚教高中。酚compounds宪法之高toxicity事业。醋酸乙基extract jiringa豆荚有高中的toxicity因为it contains酚compounds。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Talenta Conference Series: Science and Technology (ST)

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