Chemopreventive Activity of Roselle’s Hexane Fraction against Breast Cancer by In-Vitro and In-Silico Study

Abstract

Breast cancer is ranked the highest percentage of the new cases and deaths in women around the world. Cancer treatments that currently exist cause side effects that are detrimental to patients triggering the development of cancer treatment that comes from nature or herbal plants. Roselle (Hibiscus sabdariffa L.) is a plant that is known to have flavonoids which are potential as anticancer agent. This study was conducted to analyze the roselle's hexane fraction as a chemopreventive agent with an antioxidant test using the DPPH method, in-vitro cytotoxic test of T47D cancer cells line by MTT method, and in-silico test using Vina molecular docking method. The results of the antioxidant test showed the IC50 value of 4259 μg/mL. Cytotoxic activity of the fraction on T47D breast cancer cells showed the IC50 values of 331.86 μg/ml. The molecular docking results obtained a docking score that shows the binding energy of cyanidin 3-O-glucoside to EGFR and HER-2 proteins of -8.2 kcal/mol and 8.1 kcal/mol, respectively. Based on these results, it can be concluded that roselle hexane fraction has a potential as a chemopreventive agent based on its molecular docking and cytotoxic activity against T47D breast cancer cells. Keywords—Hibiscus sabdariffa; cytotoxic MTT, antioxidant DPPH, molecular docking, T47D breast cancer