GFP Introduction for RAW264.7 Cells by Combining Nanosecond High Voltage Pulse and Millisecond pulse

Abstract

Electroporation (EP) method is one of the techniques used to introduce genetic material into cells. To improve the efficiency of EP method, a pulse system using a combination of two different rectangular pulses were developed. In this system, a nanosecond high voltage pulse creates pores on cell membrane at first, and then a millisecond pulse helps genetic materials move into the inside of cells. Using this system, pulse parameters for the better efficiency of GFP (green fluorescent protein) transition for HL-60, a human promyelocytic leukemia cell, were easily found. In this study, GFP introduction for RAW264.7, a macrophage cell derived from mouse cell, was tested by this pulse system.