Quantitative Evaluation of Micro-motion of Vascular Endothelial Cells in Electrical Cell-substrate Impedance Sensing (ECIS) Method Using a Precision Mathematical Model

Abstract

We constructed a precision mathematical model for quantitative evaluation of the micro-dynamics of cultured cells measured with ECIS (electrical cell-substrate impedance sensing) system that could separately estimate cell-to-cell distance and cell-to-substrate distance. For wide applications of this method, we constructed mathematical models describing for some kinds of cell and some kinds of confluent conditions. The mathematical models of these impedances could be fit well especially from 1 kHz to 10 kHz, which were most interesting frequency range for micro-dynamic analysis in ECIS methods