Heterogeneity Studies of Wild Clarias gariepinus (Osteichthyes, Clariidae) Using SDS-Polyacrylamide Gel Electrophoresis

F. A. Ola-Oladimeji, E. Idowu, A. A. Adewumi, K. C. Fafowora
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引用次数: 1

Abstract

Abstract This study determined the genetic variations that exist in Clarias gariepinus obtained from two natural populations in Nigeria, using their serum protein profiles. A total of 51 samples of Clarias gariepinus collected from Ado-Ekiti and Ilesa were used for this experiment. Blood was extracted from the caudal vein of each individual fish and electrophoresis was performed based on standard methods. Following this, gel images were taken, scored and subjected to classical cluster analysis using Bray-Curtis similarity index. This showed the presence of variations in C. gariepinus between the studied populations and samples from Ado-Ekiti reservoir displayed more diversity than those from Ilesa. Hence, this showed the feasibility for selecting samples from Ado- Ekiti to improve culture of C. gariepinus in further breeding studies.
利用sds -聚丙烯酰胺凝胶电泳研究野生鸡尾Clarias gariepinus(骨鱼科,Clariidae)的异质性
摘要本研究利用尼日利亚两个自然种群的血清蛋白谱,确定了该种群存在的遗传变异。本实验采用在Ado-Ekiti和Ilesa采集的51份克拉丽亚斯。从每只鱼的尾静脉中提取血液,并根据标准方法进行电泳。随后,对凝胶图像进行拍摄、评分,并使用布雷-柯蒂斯相似指数进行经典聚类分析。这表明在不同种群之间存在差异,Ado-Ekiti水库的样本比Ilesa水库的样本更具多样性。因此,这表明在进一步的育种研究中,选择来自Ado- Ekiti的样品来改进加里宾鸡的培养是可行的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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