Detection and Molecular Characterization of Vibrio Parahaemolyticus in Shrimp Samples

Abstract

Methods: A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains of V. parahaemolyticus.The shrimp samples were immediately homogenized and cultured on TCBS agarand subjected to confirmatory biochemical tests. Polymerase Chain Reaction (PCR) was performed for detection of total and pathogenic V. parahaemolyticus by amplification of vp–toxR,tdh and trh genes.