Determination of vancomicin- resistant strains (VRE) with VanA and VanB enzymes among field isolates of Enterococcus spp., obtained from drinking water samples

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI:10.31073/vet_biotech36-02

T. Garkavenko, O. Gorbatiuk, T. G. Kozitska, V. Andriyashchuk, S. Dibkova

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Abstract

isolates of Enterococcus spp., to identify among them VRE-strains and to confirm the production of VanA- and VanB-enzymes. Materials and methods . 14 field isolates of Enterococcus spp. isolated from drinking water samples in the Odessa region were investigated. Sensitivity determination of experimental clinical isolates of Enterococcus spp. was performed by a disc-diffusion method using antibiotic disks according to EUCAST recommendations. Results of research and discussion . Analysis of the results of studies to determine the sensitivity of Enterococcus spp. to AMP showed that 10 strains, from the studied 14, had sensitivity to all used antibiotics, that was 71,5% of all enterococcal isolates. Polyantibiotic resistance was established in 1 strain because it was insensitive to imipenem, levofloxacin, teicoplanin, and vancomycin. Among 4 strains belonging to the VRE were identified. Among them, 2 strains confirmed the production of VanA-enzymes and in 2 strains the production of VanB-enzymes. further research to study the level of resistance to AMP at film-forming Enterococcus

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用VanA和VanB酶测定饮用水中肠球菌的万古霉素耐药菌株

鉴定其中的vre菌株，并确认产生VanA-和vanb酶。材料和方法。对敖德萨地区饮用水中分离的14株肠球菌进行了调查。根据EUCAST的建议，采用纸片扩散法对临床分离的肠球菌进行敏感性测定。研究和讨论的结果。对测定肠球菌对AMP敏感性的研究结果分析显示，在所研究的14株肠球菌中，有10株对所有使用的抗生素都敏感，占所有肠球菌分离株的71.5%。1株菌株对亚胺培南、左氧氟沙星、替柯普兰和万古霉素不敏感，对多种抗生素产生耐药。其中4株属VRE。其中2株菌株证实产生vana酶，2株菌株证实产生vanb酶。进一步研究成膜肠球菌对AMP的耐药水平

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Bulletin "Veterinary biotechnology"

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