Konservasi Paphiopedilum supardii Braem& Loeb dengan Metode Penyimpanan Biji dan Perbanyakan secara In Vitro

Abstract

Paphiopedilum supardii  Braem & Loeb is one of Slipper Orchids from Southeast Kalimantan, which is included in Appendix I of CITES. It is a rare orchid with restricted habitat endemic to Kalimantan, found growing at altitude of 600-900 m above sea level.  In Indonesia, this species is categorized as the priority species for Conservation. The aim of this research is to conserve this orchid species through seed storage method in deep freezer at temperature of -20 o C then sowing seed by  in vitro  propagation.  Seed viability test was carried out in 0 day storage (without storage) as a control, then an interval of 3 months, 6 months, 9 months and still will continue until no longer able to germinate.  Four different media cultures were used to test the germination in vitro culture.  The result showed that the seeds of  Paphiopedilum supardii  were able to germinate in 4 different media i.e. Knudson C with micro nutrient (KCA), modified Knudson C (KC), modified Vacin & Went (VW) and modified Hyponex fertilizer (HS).  Subculture for multiplication and rooting phase, based on the best germination medium. The germination test showed that Knudson’C medium with micronutrient (KCA) resulted on the best performance of green protocorm , while the other media showed the brown swollen  protocorm .  Unfortunately, KCA gave the lowest percentage of germination.  Furthermore subculture for multiplication and rooting phase was the best in Knudson C medium with addition of micronutrient (KCA) and coconut water.