Phenotypic and Genotypic Detection of Virulence Factors Affecting Proteus mirabilis Clinical Isolates

Fawad Hayat, Muddasir Khan, Muhammad Umair, Sajeela Akbar, R. Javed, Syed Hussain Shah
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Abstract

The current study attempts the phenotypic and molecular detection of the virulence factors of Proteus mirabilis clinical isolates. A total of 600 urine samples were collected from urinary tract-infected patients at Khyber Teaching Hospital, Peshawar, Pakistan. P. mirabilis isolates were identified through different biochemical tests. Virulence factors including urease production, biofilm formation, and swarming phenomenon were determined by using different markers (ureC1, rsbA, and luxS genes), identified via the polymerase chain reaction (PCR) technique. Out of the selected samples, 95 samples (15.83%) were identified as P. mirabilis. The molecular study showed that all isolates (100%) possessed the ureC gene. Whereas, 90.52% and 92.63% of the isolates gave positive results for biofilm formation (rsbA gene) and swarming phenomenon (luxS gene), respectively. The phenotypic and molecular study of P. mirabilis virulence factors provides a better understanding of how P. mirabilis infection spreads. The results could be used for prevention and improvements in its clinical treatment.
影响奇异变形杆菌临床分离株毒力因素的表型和基因型检测
本研究试图对奇异变形杆菌临床分离株的毒力因子进行表型和分子检测。在巴基斯坦白沙瓦开伯尔教学医院共收集了600份尿路感染患者的尿液样本。通过不同的生化试验鉴定出奇异假单胞菌分离株。通过聚合酶链反应(PCR)技术鉴定不同的标记(ureC1、rsbA和luxS基因),确定毒力因子包括脲酶产生、生物膜形成和蜂群现象。在所选样品中,鉴定出95份(15.83%)为奇异假单胞菌。分子研究表明,所有分离株(100%)均具有ureC基因。生物膜形成(rsbA基因)和蜂群现象(luxS基因)检测阳性率分别为90.52%和92.63%。对奇异假单胞菌毒力因子的表型和分子研究有助于更好地了解奇异假单胞菌感染的传播方式。研究结果可为预防和改善其临床治疗提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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